Interviewers often use this to get the ball rolling. The key in answering this question is to be brief (you shouldn't spend more than 2 or 3 minutes answering this) and to focus on the skills required for the job. Keep in mind how your background can help the organization.
What did you enjoy least about your last position?
Don't dwell on anything negative or bad mouth a supervisor, co-workers or a department. Even though this is a negative question, you have to turn it around and answer in a positive way. Make sure that if you name a specific task that is completely unrelated to the job you're applying for (eg. do not mention software testing or technical support). Stress that even though you didn't enjoy the task you did it well because you recognized the importance.
What are your weaknesses?
We all have them. The secret is to show you are aware of them and that you're working to overcome them. Some examples, "I have a tendency to take on too much work myself. I'm trying to solve this problem by delegating more." Don't admit to weaknesses in an area that is essential to the job you're applying for. Don't admit to a personal quality that might hamper job performance (eg. don't admit to procrastination or lack of concentration). Talk about something that is tangible and that can be changed. The interviewer wants to know whether you can evaluate yourself and take steps to improve your weaknesses.
Where do you want to be in five years?
You should show that you have some goals. This is your chance to show why you are enthusiastic about this position or company. Stick to shorter-term goals and skills you want to develop that are geared to the job.
What courses are you taking?
The key is to elaborate on how the courses are related to the job for which you are applying. You should should identify your strengths in relation to the job in your answer